Table 1.

Human IgG N-glycans captured by EDGE UPLC-HILIC-FLR profiling with various BGLs.

Peak no.a	Glycan structureb	Retention time (min)	% Glycan bindingc
			rBGL	G49N	H71E	S48K
1		3.907	84	100	61	85
2		4.561	87	94	62	88
3		5.141	61	77	42	67
4		5.349	29	87	0	42
5		5.674	36	46	0	100
6		5.895	29	50	25	38
7		6.118	32	54	26	43
8		6.369	29	49	24	38
9		6.592	17	18	13	26
10		7.601	1	0	1	1
11		8.047	7	3	5	6
12		8.337	42	67	37	49
13		8.965	9	0	8	12
14		9.859	0	0	0	1
15		12.081	0	0	0	6
16		12.34	0	0	0	6

^aA complex mixture of 2AB-labeled human IgG N-glycans was subjected to EDGE sample preparation and UPLC-HILIC-FLR profiling to visualize 16 chromatography peaks that are known to each contain single or multiple N-glycan structures.

^bSugar symbols are as shown in Fig. 2

^cThe percentage of each initial chromatography peak area that is recovered after lectin enrichment. It is calculated as described in “Materials and methods” section.