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. 2021 Jan 8;11:162. doi: 10.1038/s41598-020-80576-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Percentage of cell cluster formation on Day 5. (A) The proportion of single and double cells under different pressure conditions. (B) Proportion of cell clusters under different pressure conditions. (C) Representative image from the cell culture analyzer. Green circles indicate viable cells, and red crosses indicate dead cells based on Trypan blue exclusion. (n = 3) *Denotes statistical significance (p < 0.05). “n.s.” denotes no statistically significant difference (p > 0.05).