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. 2021 Jan 8;11:32. doi: 10.1038/s41598-020-79378-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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OCT significantly reduces secretion and activity of proteases in wounded human skin. (A) Indicated MMP levels, analysed by LC–MS, were significantly lower in human TS skin biopsies treated with OCT compared to biopsies which were untreated or treated with a control gel, with exception of TIMP-1, which is not affected upon OCT treatment (left panel). Label-free quantification (LFQ) intensities in a logarithmic scale to the basis 2 are indicated. LFQ intensities for proteins not detected in a replicate were replaced by 15. Proteases and a typical protease inhibitor in culture supernatants were tested in duplicates with an ELISA (right panel). (B) Enzymatic activity of MMPs was significantly lower in culture supernatants of OCT-treated TS human skin biopsies compared to controls (untreated, control gel-treated). Relative fluorescence units (RFU) are indicated. All data is presented as mean ± SD (n = 5). An unpaired t-test was performed with GraphPad Prism. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ns = not significant.