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. 2021 Jan 8;11:163. doi: 10.1038/s41598-020-80326-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of human cardiomyocyte (hCM) proliferation. Comparison of the MTS tetrazolium compound reduction in a colorimetric assay to asses hCM proliferation cultured for 24 or 72 h in normoxic (Normoxia) and hypoxic (Hypoxia) culture conditions with amniotic fluid stem cells secretome (AFSC-S) collected from normoxic (nAFSC-S) or hypoxic (hAFSC-S) conditions collected at 6 to 30 h. (A) Cell proliferation fold change evaluation of recorded values normalized to hCM proliferation values in control basal medium (basal MEMα; dashed line) and (B) absolute absorbance values cross parameter comparison. Data are expressed as means ± SD. n = 5. * above a bar graph indicates statistical significance between AFSC-S group and basal MEMα group. * above a line indicates statistical significance between nAFSC-S and hAFSC-S group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by two-way ANOVA followed by Tukey’s post hoc test.