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. 2021 Jan 8;11:178. doi: 10.1038/s41598-020-80644-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Reaction temperature optimization. (a) Temperature optimization (ranged between 50 to 66 °C) for the LAMP assay with Ethidium bromide and basic fuchsin dyes. (b) Further, results were visualized on a 2% agarose gel. Where lanes 1 to 9 indicate the temperature in °C (50, 52, 54, 56, 58, 60, 62, 64, and 66), lane N indicates negative control, and Lane L indicates 1 kb ladder (Thermo Scientific, USA).