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. 2021 Jan 8;11:178. doi: 10.1038/s41598-020-80644-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative analysis of PCR, qPCR, LAMP assay, and HAD for their sensitivity using Sarocladium oryzae specific primers. (a) The sensitivity of the PCR assay, the result was visualized in a 1% agarose gel. Where lane L indicates 1 Kb ladder (Thermo Scientific), lanes 1–7 indicates 10 ng, 1 ng, 100 pg, 50 pg, 10 pg, 100 fg, 50 fg, and lane N indicates negative control. (b) The sensitivity of the qPCR assay. The standard curve represents log10 of DNA concentration in fg against Ct (cycle threshold) values for different DNA concentrations. (c) The sensitivity of the LAMP assay. The result was visualized in a 2% agarose gel. Lanes (1 to 7) indicates template DNA concentrations (10 ng, 1 ng, 100 pg, 50 pg, 10 pg, 100 fg, and 50 fg); lane N indicates negative control. (d) The sensitivity of the HDA assay. The result was visualized in a 1% agarose gel. Lanes (1 to 7) indicates template DNA concentrations (10 ng, 1 ng, 100 pg, 50 pg, 10 pg, 100 fg, and 50 fg), lane N indicates negative control, and lane L indicates 1 kb ladder (Thermo Scientific).