. 2021 Jan 8;11:178. doi: 10.1038/s41598-020-80644-z

Table 1.

Oligonucleotide primers used for different assays.

For the PCR, HDA, and qPCR assays
Pathogen	Type	Primer sequence	Primer coordinates	Reference ID	Target gene
M. oryzae	Forward	AGGCTCACCACATTCTCAAG	521–539	XM_003713594	RNA polymerase II largest subunit
M. oryzae	Reverse	GTCGCCAAGCTTGTACGT	699–716	XM_003713594	RNA polymerase II largest subunit
S. oryzae	Forward	CGACAAGAAGAACCCTCGC	35–53	MW314046	RNA polymerase II largest subunit
S. oryzae	Reverse	CAGGATCTCTTTCATCCGCC	348–368	MW314046	RNA polymerase II largest subunit
For the LAMP assay
M. oryzae	F3	AGGCTCACCACATTCTCAAG	521–539	XM_003713594	RNA polymerase II largest subunit
	B3	GTCGCCAAGCTTGTACGT	699–716
	FIP	GCCATTCGGGTCTGGCATAGTC-TATTCCCGAAGACGACCTGT	FIP (F1c + F2):581–603 + 542–561
	BIP	CGTCACGGTTCTGCCAGTGC-TACGCATTCCCTGCGATG	BIP (B1c + B2):608–627 + 667–684
	LF	CTTGTTCAGGCCCATCTTCC	562–581
	LB	CCTCCCGTCAGGCCCAGTAT	633–652
S. oryzae	F3	CGACAAGAAGAACCCTCGC	27,193–27,211	MW314046	RNA polymerase II large subunit
	B3	CAGGATCTCTTTCATCCGCC	27,406–27,425
	FIP	TAGCTTGCCCACATGTTCTGCA-GAAGGACTCAACGATCCGAG	FIP (F1c + F2) (106–128 + 55–77)
	BIP	TTGCCTGCCCAGTCTATCACC-AGCAGTTGTGGCAAACAATC	BIP (B1c + B2) (264–284 + 319–338)
	LF	TACTGCCTGTCAATAGAGCC	82–101
	LB	GCTACATCAAGAAAGTCAA	288–306