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. 2021 Jan 8;11:232. doi: 10.1038/s41598-020-80291-4

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of NF-κB downregulates LPS-induced Glut1 expression in macrophages. (A,B) RAW264.7 cells were initially treated with IKKβ-inhibitor SC-514 (for 1 h) and then treated with LPS for additional 1 h (for protein analysis) and 4 h (for RNA analysis). Proteins were analyzed by western blotting using antibodies against phospho-p65 (NF-κB subunit), IκBα, Glut1, and β-actin (loading control) (panel A, quantifications in panel B). (C,D) RNA was isolated from above treated and control cells. Expressions (relative to GAPDH) of HOTAIR, and Glut1 were measured by RT-qPCR. GAPDH was used as control. Data represent mean ± SD (n = 3); *p < 0.05, **p < 0.001, ***p < 0.0001.