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. 2021 Jan 8;11:232. doi: 10.1038/s41598-020-80291-4

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Knockdown of HOTAIR reduces the LPS-dependent recruitment of NF-κB on Glut1 promoter. RAW264.7 macrophage cells were transfected with HOTAIR and scramble-siRNA, then treated with LPS (1.5 h). Cells were then fixed with formaldehyde and subjected to ChIP assay using antibodies specific to phospho-p65, CBP, histone acetylation, H3K4m3, RNA pol II and β-actin (control). The immunoprecipitated DNA fragments were analyzed by qPCR using primers specific to the NF-κB binding regions on Glut1 promoter. The location of NF-κB binding site at the Glut1 promoter is shown in the top panel. Each experiment was repeated at least thrice (n = 3). Data represent mean ± SD; *p < 0.05, **p < 0.001.