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. 2021 Jan 8;11:232. doi: 10.1038/s41598-020-80291-4

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HOTAIR regulates upstream-regulators of glucose metabolism under LPS-simulation. (A,C): RAW264.7 cells were treated with LPS for varying times. RNA was analyzed by RT-qPCR for the expression of PTEN and HIF-1α. GAPDH was used as control. Data represent mean ± SD (n = 3); *p < 0.05, **p < 0.001, ***p < 0.0001. (B,D): RAW264.7 cells were transfected (48 h) with HOTAIR-siRNA and scramble siRNA followed by treatment with LPS. RNA was analyzed by RT-qPCR for the expression of PTEN (panel B) and HIF-1α (Panel D). GAPDH was used as control. Data represent mean ± SD (n = 3); *p < 0.05, **p < 0.001, ***p < 0.0001. (E,F) Proteins from HOTAIR-siRNA and scramble siRNA treatments (48 h) followed by 1 h LPS-treated RAW264.7 cells were analyzed by Western blotting using antibodies against HIF-1α and β-actin (loading control). The changes in amounts of HIF-1α have been quantified by ImageLab5.2.1 software and shown in panel F.