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. 2021 Jan 8;11:42. doi: 10.1038/s41598-020-79779-w

Figure 3.

Figure 3

Tumor clonogenic assay (TCA). (a) TCA was performed in a 6-well plate, with clones produced by T98G and HACAT cells at day 12 following treatment with DAV and TMZ at 1 mg/mL and 0.2 mg/mL, respectively. (b) The number of colonies containing ≥ 50 cells was counted under a microscope. (c,d) Relative colony number was represented for T98G and HACAT cells, respectively. Values are shown in μg/mL. Linear mixed models to assess the effects of treatments were used as described in Fig. 1. Data are means ± SEM (n = 2).