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. 2021 Jan 8;6:5. doi: 10.1038/s41541-020-00267-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a–c Western blot of fractions from sucrose gradient purified wt VLPs of PV1 (a), PV2 (b) and PV3 (c) expressed using the PV-IRES cassette. Gradients are fractionated from top (fraction 1) to bottom (fraction 12). Detection was with anti-poliovirus blend of monoclonal antibodies MAB8566 (Millipore). d Coomassie stained gel of pooled, desalted and concentrated peak fractions for PV1 wt, PV2 wt and PV3 wt identified in a–c. Protein bands for individual subunits VP0 (37.5 kDa), VP1 (33.1 kDa) and VP3 (26.5 kDa) are labelled. e-g Negative stain EM images of PV1 wt, PV2 wt and PV3 wt VLPs produced from the PV-IRES expression cassette. Black arrowheads highlight individual VLPs. Scale bars 100 nm.