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Matrix of plasmids tested for transient expression by transfection into GMK cells. Expression of the P1 genes is regulated by the T7 promoter, with the T7 polymerase provided in trans through infection with MVA-T7, whilst that of the downstream 3CD gene is achieved by one of the three strategies listed on the left.
| PV1 | PV2 | PV3 | |
|---|---|---|---|
| FMDV-2A | pMVA-PV1_FMDV-2A | N.D. | pMVA-PV3_FMDV-2A |
| HIV-FS | N.D. | pMVA-PV2_HIV-FS | N.D. |
| PV-IRES | pMVA-PV1_PV-IRES | pMVA-PV2_PV-IRES | pMVA-PV3_PV-IRES |
