Fig. 2. Copper regulation of homeostasis systems in B. pertussis and B. bronchiseptica.

(a, c, and d) RNAseq analyses of B. pertussis grown for 16 h in the presence of 2 mM CuSO₄ in SS medium (a), B. pertussis grown in SS medium and treated for 30 min with 2 mM of CuSO₄ (c), and B. bronchiseptica grown for 12 h in the presence of 2 mM CuSO₄ (d). Comparisons were made with bacteria grown in standard conditions. Each gene is represented by a dot. The x and y axes show absolute levels of gene expression in reads per kilobase per million base pairs (RPKM) in standard and copper conditions, respectively. The genes indicated in blue indicate genes of interest with the strongest regulation factors. The full sets of data are shown in Supplementary Tables S1, S2 and S3. (b) Summary of the transcriptomic and proteomic analyses performed after growing bacteria as in (a) and (c) in medium supplemented with 2 mM CuSO₄. Standard culture conditions were used for comparisons. * indicates small proteins difficult to detect by global proteomic approaches.