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. 2021 Jan 8;4:46. doi: 10.1038/s42003-020-01580-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Representative images of THP1 macrophages having engulfed B. pertussis harboring the mRPF1 gene under the control of the copZ-prxgrx-gorB operon promoter. Macrophages were either starved of copper using a chelator or treated with copper chloride prior to contact with bacteria. The bacteria are red, cell membranes are green, and nuclei are blue. In the two fields shown at the left, the bacteria were projected as objects, showing that similar numbers are present in both conditions. All image panels are shown at the same magnification. b Levels of fluorescence (arbitrary units) of intracellular bacteria in the two conditions. Statistical analyses were performed using a two-tailed Student T-test (****, p < 0.0001). The horizontal orange lines represent median values (n > 500 in both cases).