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. 2021 Jan 8;12:204. doi: 10.1038/s41467-020-20426-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The presence of three complementary CAG (orange) and CTG (blue) repeats in the duplex and 10 CAG repeats in the slip-out (orange) could result in four positional isomers P1–P4. b 2D MFD plot shows FRET efficiency (E_FRET) or donor anisotropy (r_D) vs donor lifetime [τ_D(A)] for freely diffusing 3WJs. c MFD FRET histogram fits to six FRET states. d TIRF time traces of surface-immobilized 3WJs; Alexa488 (green) and Cy5 (red) intensities, and calculated FRET efficiency (E_FRET). e TIRF FRET histogram fitted to six FRET states. Measured at 20 °C in a buffer containing 1 mM MgCl₂.