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. 2021 Jan 8;12:204. doi: 10.1038/s41467-020-20426-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Transition density plots from TIRF time traces of 3WJs with three complementary CAG (orange) and CTG (blue) repeats in the duplex and (CAG)_n slip-outs (n = 4, 6, 8, 10, 20, and 30). 3WJs with slip-outs of (CAG)₄ have two FRET states, while all other 3WJs are fitted to six FRET states. Similar plots are found for 3WJs with (CTG)_n slip-outs (Supplementary Fig. 20). Measured at 20 °C in a buffer containing 1 mM MgCl₂.