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. 2021 Jan 8;12:149. doi: 10.1038/s41467-020-20375-x

Fig. 1. Timeline of key discoveries and milestones of resilin and RLP-based biomaterials.

Fig. 1

Description of illustrations (in the order of year): 1960—locust-wing hinge showing resilin, 1964—covalent dityrosine crosslinks in native resilin, 1966—native uncrosslinked pro-resilin synthesized by epidermal cells into the subcuticular space, 2001—full-length resilin (fruit fly) showing the three exon regions with respective sequence length, 2005—RLPs synthesis by recombinant technology, 2009—domain selection and design of modular RLP, 2011 (top)—RLP (antigen) and RLP-antibody interaction for bioimaging, 2011 (bottom)—plot (temperature versus hydrodynamic diameter) showing both upper critical solution temperature (UCST) and lower critical solution temperature (LCST), dual-phase behaviour of RLPs, 2012—structural conformation of full-length resilin to stress, 2013—crosslinked network between RLPs (blue) and four-arm polyethylene glycol (PEG; green) hybrid hydrogel, 2015—plot (temperature versus ultra-violet absorbance) showing soluble to insoluble transition of RLPs in aqueous solution and 2018—resilin gene expression in plants.