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. 2021 Jan 8;11:157. doi: 10.1038/s41598-020-79592-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence images of fCNC-AF488 in mouse (A,B) heart, (C,D) kidney, (E,F) liver and (G,H) spleen tissue. Anti-AF488 staining (green) 24 h after intravenous administration of fCNC-AF488. Tissue distribution of nanomaterial was not evidenced in heart tissue but accumulation was noted in kidney, liver and spleen. Scale bars are for Panels (A,C,E,G) are 75 µm; scale bars are for Panels (B,D,F,H) are 25 µm. Nuclei (blue) are DAPI-stained.