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. 2021 Jan 8;11:53. doi: 10.1038/s41598-020-79693-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Live(green)/dead(purple) stain of MCF7 cells cultured under hypoxic conditions as a spheroid (a–c) and as an adherent monolayer (d–f) after 48 h. The seeding number increases from left to right with 1250 cells (a,d), 1800 cells (b,e), and 2500 cells (c,f) in 2.5 $μ$ L. Scale bar is the same for all the micrographs in this figure.