Figure 2.

Pre-recall NMDAR blockade does not affect SDIA extinction memory but impedes the amnesic effect of reconsolidation inhibition. (a) Animals were trained in SDIA (TR; 0.4 mA/2 s) and beginning 24 h later they were submitted to one daily extinction training trial for 5 consecutive days. One day after the last extinction training trial, animals received bilateral intra-CA1 infusions of vehicle (VEH; 1% DMSO in saline) or the NMDAR antagonist AP5 (5 µg/side), and 20 min thereafter, SDIA extinction memory was reactivated (RA). Retention was assessed 1 day and 7 days later (Test). (b) Animals were treated as in A except that they received intra-CA1 infusions of VEH or rapamycin (RAP; 0.02 µg/side), an inhibitor of mammalian target of RAP (mTOR) 5 min after RA. (c) Animals were treated as in A and ,5 min after RA, they received bilateral intra-dorsal CA1 infusions of VEH or RAP. (d) Animals trained in SDIA received bilateral intra-CA1 infusions of VEH or AP5 one day post-training and, 20 min later, were submitted to a SDIA memory retention test. Data are expressed as median ± IQR. (**) p < 0.01, (***) p < 0.001 versus VEH in Dunn's multiple comparisons after Kruskal–Wallis test; n = 9–12 animals per group.