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. 2021 Jan 8;11:61. doi: 10.1038/s41598-020-79732-x

Table 1.

Sperm motility and egg:sperm ratios for fresh, DMSO-treated, and cryopreserved sperm in three volumes (50, 100, and 200 µL) used for fertilisation.

	Fresh sperm	DMSO sperm	Cryo sperm
Total motility (%)	54	65	6
Motile concentration	2.4 × 10⁷ cells/mL	1.1 × 10⁷ cells/mL	2.0 × 10⁶ cells/mL
Egg: motile sperm ratio
50 µL sperm	7.4 × 10³ cells/egg	2.8 × 10³ cells/egg	8.4 × 10² cells/egg
100 µL sperm	1.4 × 10⁴ cells/egg	5.5 × 10³ cells/egg	1.2 × 10³ cells/egg
200 µL sperm	3.4 × 10⁴ cells/egg	9.6 × 10³ cells/egg	3.0 × 10³ cells/egg