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. 2021 Jan 8;11:40. doi: 10.1038/s41598-020-79524-3

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021

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Up-regulated expression of HIF1α, GPC3, and FAT1 under hypoxia. (A) HIF1α mRNA level in HepG2 cells after DP (100 µM) treatment for different lengths of time. (B) GPC3 and (C) FAT1 mRNA levels after same treatment as (A). (D) Western blot analysis of HIF1α, GPC3, and FAT1 protein levels in HepG2 cells after DP (100 µM) treatment. Full-length blots/gels were presented in Supplementary Figure S4. Quantification was performed based on the band intensity and presented in (E) HIF1α, (F) GPC3, and (G) FAT1. Data represent mean ± SEM (n = 3). **P < 0.01; ***P < 0.001. ns, not significant.