Table 4.

Summary of immunohistochemistry for pig retinal degeneration model.

	Sequence of antibody (primary/secondary)	Dilution	Industry	Targeted cells or layers
Anti-PKC-α	Primary	1:200	Invitrogen	(rod) Bipolar cells. Targeted protein kinase (PKC)-α is abundant in retinal bipolar cells
Anti-GFAP	Primary	1:300	NOVUSBIO	Müller cells, astrocytes, and glical cells. Targeted GFAP is a member of the class III intermediate filament protein family abundant in astrocytes and glial cells
Anti-NeuN	Primary	1:250	Merck	Retinal ganglion cells. Targeted NeuN is DNA-binding, neuron-specific protein
Anti-PNA	Primary	1:300	VECTOR	Cone cells. Targeted S opsin exists in cone cells
Anti-rhodopsin	Primary	1:200	Rockland	Rod cells. Targeted retinal rhodopsin exists in rod cells
Anti-RPE65	Primary	1:200	Invitrogen	RPE cells. RPE65 is a major protein of the RPE
Alexa Fluor 594-conjugated goat anti-mouse	Secondary	1:300	Biogend	Capture or detection of mouse IgG in ELISA

PKC-α Protein kinase-α, GFAP Glial fibrillary acidic protein, NeuN Neuron-specific nuclear protein, PNA peanut agglutin, RPE retinal pigment epithelium, IgG Immunoglobulin G, ELISA Enzyme-linked immunosorbent assay.