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. 2021 Jan 8;11:228. doi: 10.1038/s41598-020-80546-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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SE-ADM images of 4T1E/M3 cells 3 and 24 h after the addition of PM2.5. (a) A low magnification (1200×) image of 4T1E/M3 cells 3 h after the addition of PM2.5. A number of PM2.5 aggregates were seen in the whole visual field. (b, c) High magnification (b 10,000×; c 20,000×) images of the areas indicated by red arrows in (a). The images show high-density black particulate aggregates covered with light intracellular membrane-like structures. (d) A low magnification (1000×) image of 4T1E/M3 cells 24 h after the addition of PM2.5. (e, f) High magnification (10,000 ×) images of the areas indicated by red arrows in (d). Scale bars, 10 μm in (a, d), 1 μm in (b, e, f), and 500 nm in (c).