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. 2021 Jan 8;11:62. doi: 10.1038/s41598-020-79660-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Lipid profiles of worker jelly samples (A) and larvae (B, C) from week 3. Samples of worker jelly (A) were taken using absorptive filter strips (Sugi strips), extracted with n-hexane and separated by HPTLC. Each Sugi strip was halved: one half without the sample (track a) and the other with absorbed sample (track b). A fresh Sugi strip from the same batch as the strips used in the experiment cut in two equal halves served as the blank. The lanes are labeled to show the dose of clothianidin treatments (control = no clothianidin). The analysis of larvae is shown before (B) and after derivatisation (C). For worker jelly, the declining quantity of substances was observed for the lipid profiling (UV 366 nm after derivatization). The latter also was evident for the larvae. Numbers on the right side indicate differences, see SFig 3B, F and H.