Fig. 3.

HCMV IE1 and IE2 protein expression following LC-1310 treatment. Expression of IE1 and IE2 proteins during HCMV infection in the presence or absence of p97 inhibitor LC-1310. Parallel HFF cultures were inoculated, compounds added and cells processed for western blotting each day post-inoculation. The cell lysates were separated by polyacrylamide gel electrophoresis on a 10% gel and then transferred to a PVDF membrane. IE1 and 2 are detected using an anti-HCMV IE1/IE2 monoclonal antibody. The β-actin levels were detected using a rabbit anti-β-Actin monoclonal antibody and are shown as a loading and expression control