Fig. 5.
LC-1310/LTV combination effects. a Anti-HCMV activities of the combinations. HFFs were inoculated as described in the HCMV replication assay and compound combinations applied. Six days later, GFP fluorescence was measured as an indication of virus replication and spread. Inhibitory effects of combinations were expressed as a percentage of the DMSO-alone samples. All compounds doses were performed in duplicate and the mean value plus standard error depicted. b The viability of HFFs following compound combination treatment for 6 days was determined by the MTS viability assay and presented as a percentage of the viability of cells that were treated with DMSO alone. c Three-dimensional surface plots of LC-1310 and Letermovir combinations and anti-HCMV activities are shown. The three-dimensional plots were generated and interpreted as described in Fig. 4 legend. Blue color = −10 to 0 and green = 0–10 μM2% for a given combination