Figure 2.

Protein expression levels of estrogen synthesis enzyme HSD17β1 and receptor GPER1 were analyzed by Western blot analyses and immunofluorescence staining. (a) Estrogen enzyme HSD17β1 protein expression level in lysates from indicated cultured cells was examined by Western blot analysis and the density of blot bands is quantified (b); (c) estrogen receptor GPER1 protein expression level in cultured melanocytes was examined by immunofluorescence staining with anti-GPER1 antibody (green) and Hoechst 33342 (blue). White bar, 50 µm. Data in (b) represent the results of three independent experiments. Data are shown as means ± SD.