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. 2020 Dec 30;22(1):285. doi: 10.3390/ijms22010285

Figure 5.

Figure 5

IL-36 (α, β, and γ) induced expression of angiogenic factors. (A) HTR-8/SVneo and primary trophoblast cells (PTC) were stimulated with 20 or 50 ng/mL IL-36 (α, β, and γ) for 24 h. The mRNA levels of VEGFA and PGF were determined by quantitative real-time PCR and normalized to GAPDH using the 2−ΔCt method. Results from three independent experiments are shown as mean ± SEM. NTC: Non-treated cells. Unpaired two-tailed Student’s t-test with Welch´s correction. * p < 0.05, ** p < 0.01 for significant differences to NTC controls. (B) VEGFA, PGF, and GAPDH proteins were detected by Western blotting using 20 μg cell lysate from HTR-8/SVneo and PTC stimulated for 24 h.