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. 2020 Dec 30;13(1):85. doi: 10.3390/cancers13010085

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Biochemical characterization of the affibody-drug conjugates (AffiDCs). (A) Schematic description of the AffiDCs and the non-toxic control Z_HER2:2891-ABD-E₃-AA. A G₄S linker was used to connect Z_HER2:2891 domains and the albumin binding domain (ABD) in all constructs. (B) SDS-PAGE analysis under reducing conditions. The numbers to the left correspond to the molecular weight in kDa of marker proteins in lane M. (C) Size-exclusion chromatography analysis. The numbers above the chromatograms corresponds to the elution volumes of protein standards with the indicated molecular weights (kDa). (D) RP-HPLC analysis during a 20 min linear gradient from 30% to 60% acetonitrile in water supplemented with 0.1% TFA.