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. 2020 Dec 30;13(1):85. doi: 10.3390/cancers13010085

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cell binding and cellular processing of ^99mTc-labeled AffiDCs. (A) SKOV3 and BT474 cells were incubated with radiolabeled AffiDC with or without pre-saturation with a 500-fold molar excess of the same AffiDC lacking radiolabel, blocked or non-blocked, respectively. The Y-axis corresponds to the measured total radioactivity of the cells as a percentage of the total radioactivity added to each well. (B) Cellular processing of 2 nM conjugates by SKOV3 and BT474 cells during continuous incubation over 24 h. Data were normalized to the recorded value at 24 h, which was set to 100%, and are presented as mean with error bars corresponding to 1 SD. (C) Interaction maps for the interaction between ^99mTc labeled AffiDCs and SKOV3 cells. The maps were derived by InteractionMap software from real-time biosensor measurements in a LigandTracer instrument. The x-axis corresponds to the dissociation rates and the y-axis corresponds to the association rates of the interactions in each graph.