Table 2.

Kinetic parameters and equilibrium dissociation constants.

Analyte	Ligand	ka (M−1·s−1)	kd (s−1)	KD (M)
ZHER2:2891-ABD-E3-mcDM1	HER2	2.88 × 105	2.10 × 10−4	7.28 × 10−10
	HSA	1.34 × 106	7.50 × 10−5	5.58 × 10−11
	MSA	2.35 × 106	2.07 × 10−3	8.97 × 10−10
ZHER2:2891-ABD-G3-mcDM1	HER2	4.04 × 105	2.26 × 10−4	5.59 × 10−10
	HSA	1.09 × 106	5.95 × 10−5	5.46 × 10−11
	MSA	1.92 × 106	1.31 × 10−3	6.81 × 10−10
(ZHER2:2891)2-ABD-E3-mcDM1	HER2	ND a	ND	ND
	HSA	1.51 × 105	2.29 × 10−5	1.51 × 10−10
	MSA	2.08 × 105	2.03 × 10−3	9.78 × 10−9
ZHER2:2891-ABD-E3-AA	HER2	8.09 × 105	2.38 × 10−4	2.94 × 10−10
	HSA	1.73 × 106	6.69 × 10−5	3.87 × 10−11
	MSA	2.82 × 106	2.07 × 10−3	7.34 × 10−10

^a Not determined.