Figure A2.

Gene expression analysis of scaffold-free cartilage components for SOX9, COL2A1, COL10A1 ACAN, RUNX2 and COL1A1 were performed after 0, 7, 14 and 21 days. The relative gene expression was normalized to the housekeeper gene EF1A and day 0. Data are shown as mean ± SEM (duplicates per gene) for n = 3. Mann-Whitney U-test was used to determine the statistical significance. SOX9, SRY-box transcription factor 9; COL2A1, collagen type II alpha 1; COL10A1, collagen type X alpha 1; ACAN, aggrecan; RUNX2, runt-related transcription factor 2; COL1A1; collagen type I alpha 1; EF1A, eukaryotic translation elongation factor 1 alpha.