Table 2.
Primary and secondary antibodies used for immunofluorescence staining.
|
Primary
Antibody |
Dye | Host | ID | Concentration [mg/mL] | Company |
| Phalloidin | TRITC | - | P1951 | 1.5 | Sigma‑Aldrich |
| Laminin | - | rabbit | NB300 | 0.8 | Novus Biologicals LLC |
| Osteopontin | A488 | rabbit | ab8448 | 1 | Abcam |
| Collagen 1 | - | mouse | ab6308 | 1.5 | Abcam |
| Collagen 2 | - | mouse | CO072 | 1 | Quartett |
|
Secondary
Antibody |
Dye | Host | ID | Concentration [mg/mL] | Company |
| Anti-mouse | Biotin | horse | BA-2000 | 1.5 | Vector Laboratories Inc. |
| Anti-rabbit | A488 | goat | A32731 | 2 | Thermo Fisher Scientific |