Table 3.
Sequences of primers, fragment size and GenBank ID used for qPCR.
| Gene | Sequence of Forward Primer | Sequence of Reverse Primer | GenBank ID |
|---|---|---|---|
| EF1A | GTTGATATGGTTCCTGGCAAGC | TTGCCAGCTCCAGCAGCCT | NM_001402.5 |
| RUNX2 | TTACTTACACCCCGCCAGTC | TATGGAGTGCTGCTGGTCTG | NM_001015051.3 |
| SPP1 | GCCGAGGTGATAGTGTGGTT | TGAGGTGATGTCCTCGTCTG | NM_001251830.1 |
| COL1A1 | CAGCCGCTTCACCTACAGC | TTTTGTATTCAATCACTGTCTTGCC | NM_000088.3 |
| ON | ACCAGCACCCCATTGACG | AGGTCACAGGTCTCGAAAAAGC | NM_001309443.1 |
| SOX9 | CGCCTTGAAGATGGCGTTG | GCTCTGGAGACTTCTGAACGA | NM_000346.3 |
| PPARG2 | CAAACCCCTATTCCATGCTGTT | AATGGCATCTCTGTGTCAACC | NM_015869.4 |
| COL2A1 | GTGGGGCAAGACTGTTATCG | AGGTCAGGTCAGCCATTCAG | NM_033150.3 |
| COL10A1 | CCAGCACGCAGAATCCATCT | TATGCCTGTGGGCATTTGGT | NM_000493.4 |
| ACAN | AACGCAGACTACAGAAGCGG | GGCGGACAAATTAGATGCGG | NM_001369268.1 |
| MMP1 | CTCTGGAGTAATGTCACACCTCT | TGTTGGTCCACCTTTCATCTTC | NM_001145938.2 |
| MMP3 | ATCCTACTGTTGCTGTGCGT | CATCACCTCCAGAGTGTCGG | NM_002422.5 |
| MMP13 | TCCTGATGTGGGTGAATACAATG | GCCATCGTGAAGTCTGGTAAAAT | NM_002427.4 |
| TNF | GTCTCCTACCAGACCAAG | CAAAGTAGACCCTGCCCAGACTC | NM_000594.4 |
| IL6 | TACCCCCAGGAGAAGATTCC | TTTTCTGCCAGTGCCTCTTT | NM_001371096.1 |
| IL8 | GAATGGGTTTGCTAGAATGTGATA | CAGACTAGGGTTGCCAGATTTAAC | NM_000584.4 |
| LDHA | ACCCAGTTTCCACCATGATT | CCCAAAATGCAAGGAACACT | NM_005566.4 |
| VEGFA | AGCCTTGCCTTGCTGCTCTA | GTGCTGGCCTTGGTGAGG | NM_001025366.3 |