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. 2020 Dec 28;26(1):89. doi: 10.3390/molecules26010089

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of FA, t-FEF77, and c-FEF77 on oxidative stress in HECV cells. (A) HECV cell viability after a 2 h treatment with H₂O₂; (B) Viability of HECV cells pretreated with 12.5 μM polyphenols (FA, t-FEF77, and c-FEF77) for 24 h and subsequently with H₂O₂ for 2 h. (C) Reactive oxygen species (ROS) in HECV cells were underlined by 2′,7′–dichlorofluorescin diacetate (DCF-DA) stain in the lower panel (upper panel shows the corresponding bright-field; bar: 50 μm). Data are expressed as percent vs. control (0). Values are mean ± S.D from at least three independent experiments. Significant differences are denoted by symbols on bars (*** p ≤ 0.001 vs. control; ### p ≤ 0.001 vs. H₂O₂).

Effects of FA, t-FEF77, and c-FEF77 on oxidative stress in HECV cells. (A) HECV cell viability after a 2 h treatment with H₂O₂; (B) Viability of HECV cells pretreated with 12.5 μM polyphenols (FA, t-FEF77, and c-FEF77) for 24 h and subsequently with H₂O₂ for 2 h. (C) Reactive oxygen species (ROS) in HECV cells were underlined by 2′,7′–dichlorofluorescin diacetate (DCF-DA) stain in the lower panel (upper panel shows the corresponding bright-field; bar: 50 μm). Data are expressed as percent vs. control (0). Values are mean ± S.D from at least three independent experiments. Significant differences are denoted by symbols on bars (*** p ≤ 0.001 vs. control; ### p ≤ 0.001 vs. H₂O₂).