Figure 4.

INCB057643 changes the inflammatory microenvironment in the pancreas of KC mice. LSL-Kras^G12D/+; Pdx-1-Cre (KC) mice were treated with INCB057643 7 times a week, for 1 week, starting at 9 weeks of age. Blood, pancreas, and spleen were collected. (A) Experimental design, n = 9-10 mice/cohort. (B) CD45⁺ immune cells, CD45⁺, CD3⁺ (CD3 T cells), CD45⁺, CD3⁺, CD4⁺ (CD4 T cells), CD45⁺, CD3⁺, CD4⁺, CD25⁺ (activated CD4 T cells), and CD45⁺, CD3⁺, CD8⁺ (CD8 T cells) were analyzed by flow cytometry in the pancreas. (C) Levels of COX2, p-STAT3 and α-SMA in pancreas extracts were determined by western blot. For quantitation of the blots, INCB057643-treated samples were normalized to controls. Graph bar represents 6–10 mice/group. Uncropped Western Blot Images are shown in Figure S9.