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. 2020 Dec 30;22(1):327. doi: 10.3390/ijms22010327

Figure 4.

Figure 4

Myosin IIB and VI support release-ready SV restoration. Presynaptic neurons were transfected with control, myosin IIA-, IIB-, or VI-siRNA 2–3 days before the EPSP recordings. (A) Myosin VI, but not IIB, knockdown reduced release-ready SVs after AP-evoked transmitter release. The averaged paired-EPSP ratio was plotted against the paired-AP interval. (Bar: SEM * p < 0.05; Bonferroni post hoc test after one-way ANOVA. VI- knockdown vs. control IIA- or IIB- knockdown) (B) Myosin IIB and VI knockdown impaired transmitter release during APs firing at 10 Hz, resulted in a sudden reduction in third and second EPSP amplitude, respectively. (C) Myosin IIB and VI knockdown delayed recovery from the RRP depletion. After a 1 min control recording at 1 Hz, a 4-min AP train at 5 Hz was applied to deplete SVs. EPSP amplitudes were normalized to the mean EPSP amplitudes before the 4-min train. Recovery rate of release-ready SVs is estimated by double exponential growth fit to the increase in averaged EPSP amplitude after the depletion. +Dyn1: double knockdown of dynamin-1 and myosin; +Dyn3: double knockdown of dynamin-3 and myosin.