Figure 2.

Klf10 deletion impairs triglyceride package and secretion upon high-sucrose feeding. Eight-week-old WT and Klf10 KO mice were fed either CD or HSD for eight weeks (n = 5–6 mice/group). Liver genes and proteins involved in (A) lipogenesis (fatty acid synthase, FAS), fatty acid uptake (fatty acid translocase, CD36), and (B) lipid secretion (microsomal triglyceride transfer protein, MTTP, and Apolipoprotein B, ApoB) were analyzed via qPCR and Western blotting. (C) Plasma triglyceride, cholesterol, HDL-cholesterol (HDL-C), and LDL-cholesterol (LDL-C) were measured. (D) In vivo VLDL secretion from livers of mice was determined. Eight-week-old WT and Klf10 KO mice were fed either CD or HSD for one week and then injected with poloxamer 407 (P-407) (n = 6 mice/group). Blood was collected at the indicated time points, and TG was measured. All data are representative of at least three independent experiments and expressed as mean ± SEM. Charts were produced using GraphPad Prism 5.0. Statistical differences were determined by two-way ANOVA with Mann–Whitney U test using SPSS v17.0. * p < 0.05 vs. genotype-matched, CD-fed group and # p < 0.05 vs. diet-matched, genotype control. WT, wild type; KO, knockout; CD, control chow diet; HSD, high-sucrose diet.