Figure 3.

Klf10 deletion induces oxidative stress upon high-sucrose feeding. Eight-week-old WT and Klf10 KO mice were fed either CD or HSD for eight weeks (n = 5–6 mice/group). Changes in hepatic mRNA and protein expression involved in oxidative stress response were analyzed via (A) qPCR and (B) Western blotting. Antioxidant genes (Nrf2, Nqo1, and Srxn1) and ROS scavenger genes (Catalase and Sod2) were analyzed. Target gene expression was normalized to the expression of Rplp0. (C) Representative 8-oxo-2′-deoxyguanosine (8-oxo-dG)-stained livers (Scale bars: 60 μm, Magnification: 20×). All data are representative of at least three independent experiments and expressed as mean ± SEM. Charts were produced using GraphPad Prism 5.0. Statistical differences were determined by two-way ANOVA with Mann–Whitney U test using SPSS v17.0. * p < 0.05 vs. genotype-matched, CD-fed group and # p < 0.05 vs. diet-matched, genotype control. WT, wild type; KO, knockout; CD, control chow diet; HSD, high-sucrose diet.