Figure 3.

Tif51A expression drops during glucose exhaustion but recovers in a Hap1-dependent manner in the post-diauxic phase. (a) The WT and hap1∆ cells were grown in YPD medium for 96 h and samples were collected at the indicated time points. (b–f). Relative TIF51A (b), eIF2A (c), SDH1 (d), HAP4 (e) and HAP1 (f) mRNA levels were determined. (g,h). A representative Western blotting experiment (g) and quantification analysis (h) of the eIF5A and eIF2A protein levels in the WT cells at the indicated time points. G6PDH protein levels were used as loading controls. The results are shown as the means ± SD of three independent experiments and are expressed in relation to the value at time 0. Statistical significance was measured by a Student’s t-test in relation to time 0. * p < 0.05, ** p < 0.01, *** p < 0.001.