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. 2020 Dec 28;22(1):219. doi: 10.3390/ijms22010219

Figure 5.

Figure 5

eIF5A expression is regulated by the metabolic flux into the TCA cycle and heme cellular levels. (a,b) The WT, hap1∆, mpc1∆ and pda1∆ strains were grown in YPGal medium for 24 h. The relative TIF51A (a) and SDH1 (b) mRNA levels were determined. The results are expressed in relation to the WT value. (c–e) The WT cells were grown in YPD medium with or without the addition of ALA (300 µg/mL) or hemin (25 µg/mL) for 24 h. The relative TIF51A (c), SDH1 (d) and HAP1 (e) mRNA levels were determined. The results are expressed in relation to the value for the no supplement condition. (a–e) and are shown as the means ± SD of three independent experiments. Statistical significance was measured by a Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001.