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. 2020 Dec 24;22(1):113. doi: 10.3390/ijms22010113

Table 2.

Oligonucleotide primer sequences for quantitative reverse transcriptase polymerase chain reaction.

Gene Primer Sequence (Forward) Primer Sequence (Reverse) Annealing Temperature (°C) Product Size (bp) RefSeq Number
IL6 5′-AGAGACTTCCAGCCAGTTGC-3′ 5′-TGAAGTCTCCTCTCCGGACT-3′ 60 88 NM_012589.2
IL1β 5′-CACCTTCTTTTCCTTCATCTTTG-3′ 5′-GTCGTTGCTTGTCTCTCCTTGTA-3′ 60 241 NM_031512.2
NFκB 5′-TGTCTGCACCTGTTCCAAAGA-3′ 5′-TGCCAGGTCTGTGAACACTC-3′ 60 143 NM_199267.2
CYP1A1 5′-CATCCCCCACAGCACCATAA-3′ 5′-TTCGCTTGCCCAAACCAAAG-3′ 60 212 NM_012540.2
GAPDH 5′-ATTGTTGCCATCAACGACCC-3′ 5′-TGACTGTGCCGTTGAACTTG-3′ 60 94 NM_017008.4