Table 2.
Gene | Primer Sequence (Forward) | Primer Sequence (Reverse) | Annealing Temperature (°C) | Product Size (bp) | RefSeq Number |
---|---|---|---|---|---|
IL6 | 5′-AGAGACTTCCAGCCAGTTGC-3′ | 5′-TGAAGTCTCCTCTCCGGACT-3′ | 60 | 88 | NM_012589.2 |
IL1β | 5′-CACCTTCTTTTCCTTCATCTTTG-3′ | 5′-GTCGTTGCTTGTCTCTCCTTGTA-3′ | 60 | 241 | NM_031512.2 |
NFκB | 5′-TGTCTGCACCTGTTCCAAAGA-3′ | 5′-TGCCAGGTCTGTGAACACTC-3′ | 60 | 143 | NM_199267.2 |
CYP1A1 | 5′-CATCCCCCACAGCACCATAA-3′ | 5′-TTCGCTTGCCCAAACCAAAG-3′ | 60 | 212 | NM_012540.2 |
GAPDH | 5′-ATTGTTGCCATCAACGACCC-3′ | 5′-TGACTGTGCCGTTGAACTTG-3′ | 60 | 94 | NM_017008.4 |