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. 2021 Jan 4;22(1):437. doi: 10.3390/ijms22010437

Figure 1.

Figure 1

Auxin response reporter expression in and genetic interactions between cpi1-1, pin2-T, and aux1-T mutant combinations. (A,B) DR5:GUS expression patterns in 5-day-old WT, cpi1-1, pin2-T, cpi1-1 pin2-T, aux1-T, cpi1-1 aux1-T, aux1-T pin2-T, and cpi1-1 aux1-T pin2-T cotyledons (A) and seedling roots (B). Shown are representative images of three independent experiments (employing 10 to 16 seedlings per experiment); (C) Quantitative DR5:GUS activity assay in 5-day-old seedlings. The presented data are means ± SD of n = 3 independent experiments. * p < 0.05, ** p < 0.01 (one-way ANOVA with Tukey’s test); (D,E) Phenotypes (D) and root length (E) of 7-day-old seedlings. The presented data are means ± SD of n = 3 independent experiments (employing 9 to 37 seedlings per experiment). No significant difference between cpi1-1 single and cpi1-1 pin2-T and cpi1-1 aux1-T double and cpi1-1 aux1-T pin2-T triple mutants by Student’s t-test (one-tailed, two-sample equal variance, p < 0.05). Bars = 400 μm in (A), 100 μm in (B), and 2 mm in (D).