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. 2021 Jan 4;22(1):437. doi: 10.3390/ijms22010437

Figure 5.

Figure 5

Cycloeucalenol enhances auxin biosynthesis genes and DR5:GUS auxin response reporter expression. (A–D) Expression patterns of ProTIR2:GUS in shoots (A,C) and root tips (B,D) of 5-day-old WT seedlings grown on MS medium containing 0.1% acetone solvent (mock) or 1 μM cycloeucalenol. Shown are representative images of three independent experiments (employing 11 to 26 seedlings per experiment); (E–J) Expression patterns of ProYUC8:GUS (E,F), ProYUC9:GUS (G,H), and DR5:GUS (I,J) in root tips of 5-day-old WT seedlings grown on MS medium containing 0.1% (v/v) acetone solvent (mock) or 1 μM cycloeucalenol. Shown are representative images of three independent experiments (employing 10 to 26 seedlings per experiment); (K) GUS activity of ProTIR2:GUS, ProYUC8:GUS, ProYUC9:GUS, and DR5:GUS 5-day-old seedlings. The presented data are means ± SD of n = 3 independent experiments. * p < 0.05, ** p < 0.01 (Student’s t-test, one-tailed, two-sample equal variance). Bars = 400 μm in (A,C) and 100 μm in (B,D–J).