Figure 2.

Differentially methylated region (DMR) and differentially hydroxymethylated region (DhMR) containing genes are enriched in genes associated with carcinogenesis and gastrointestinal disease. (A) A schematic illustration of the reduced representation bisulfite sequencing (RRBS) and oxidative RRBS (oxRRBS) techniques: 5mC and 5hmC fractions are calculated through a comparison of the RRBS and oxRRBS datasets. (B,C) Heatmaps showing 1606 DMRs and 3011 DhMRs that have been identified through RRBS combined with oxRRBS. (D,E) Functions and diseases predicted to be affected by genes containing DMRs (D) and DhMRs (E), using Fisher’s exact test at a significance threshold of p = 0.05, via Ingenuity Pathway Analysis (IPA).