Skip to main content
. 2021 Jan 5;22(1):449. doi: 10.3390/ijms22010449

Figure 5.

Figure 5

Modulation of cytokine responses through TXA in resting and activated macrophages. (a) qRT-PCR expression analysis for the indicated genes in bone marrow-derived macrophages at day 7 of differentiation, stimulated with TXA (1 mg/mL) for 6 h. (b) qRT-PCR expression analysis for the indicated genes in bone marrow-derived macrophages at day 7 of differentiation, activated with 1 μg/mL LPS and simultaneously co-stimulated with TXA (1 mg/mL) for 6 h. (c) MTT proliferation assay of bone marrow-derived macrophages stimulated with the indicated concentrations of TXA. (d) Representative images of macrophage migration assays using Ibidi chambers of the indicated groups and time points. Scale bars = 50 μm. Blue lines indicate spreading cell fronts. The quantification of cell migration is shown on the right. For (a–d), n = 3–5 independent macrophage cultures per group were used as indicated with individual data points. Data presented are means ± SD. Gene abbreviations: interleukin-1 alpha (Il1a), interleukin-1 beta (Il1b), interleukin-4 (Il4), interleukin-6 (Il6), interleukin-10 (Il10), tumor necrosis factor alpha (Tnfa), CC-chemokine ligand 5 (Ccl5), cluster of differentiation 14 (Cd14), interleukin-1 receptor antagonist (Il1ra), inducible NO synthase (iNos), toll-like receptor 4 (Tlr4), transforming growth factor beta (Tgfb).