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. 2020 Dec 31;22(1):369. doi: 10.3390/ijms22010369

Figure 1.

Figure 1

Primary retinal ganglion cells (RGC) and R28 cells express PEDF-R and laminin receptor (LR). (A) Total RNA was extracted from RGC (1) and R28 cells (2) and mRNA expression was examined by reverse transcription (RT)-PCR. For comparison, mRNA expression of both PEDF receptors in rat Müller (3), ARPE-19 (4) and human retinal pigment epithelium (RPE) cells (5) is demonstrated (NC: negative PCR control). (B) Western blot analysis revealed PEDF-R and LR expression in human RPE (1), ARPE-19 (2), rat Müller (3) and R28 cells (4). Blots were probed with antibodies against PEDF-R and LR. (C) Immunofluorescence of RGC and R28 cells generated by antibody labeling was detected using microscopic imaging (red fluorescence: PEDF-R and LR). Negative controls using non-immune mouse IgG1 or rabbit IgG were included. Cell nuclei were labeled with DAPI (blue fluorescence). Scale bars, 10 µm.