Figure 2.

Soluble factors secreted by Müller cells enhance expression of PEDF receptors in R28 cells. R28 cells were cultured for 24 h under normoxia or hypoxia (0.2% O₂), in the presence (NCM or HCM) or absence (“medium”) of Müller-cell conditioned media. The effect of these media on PEDF-R and LR (A) mRNA and (B) total protein expression was determined by (A) semi-quantitative real-time PCR and (B) Western blotting, respectively. Basal expression levels (dashed lines) relate to R28 cells cultured under normoxia in basal medium. Data in (A,B) are each derived from two independent experiments and (A) are expressed as means ± standard deviation and (B) summarize Western blotting analyses of PEDF receptor to β-actin protein expression ratios. Experiments were performed with conditioned media (1–4) derived from Müller cells of four different retinae (NCM: normoxia-conditioned media; HCM: hypoxia-conditioned media).